AFDye 405 Azide

貨號
規格
價格
品牌
貨期
1307-1
1 mg
3380.0
ClickChemistryTools
現詢
1307-5
5 mg
10180.0
ClickChemistryTools
現詢
1307-25
25 mg
27900.0
ClickChemistryTools
現詢

Abs/Em Maxima: 402/424 nm

Extinction Coefficient: 35,000

Flow Cytometry Laser Line: 405 nm

Microscopy Laser Line: 405 nm

Spectrally Similar Dyes: Alexa Fluor? 405, CF? 405, Cascade Blue?, DyLight? 405

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AZDye? 405 Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, blue-fluorescent probe with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AZDye? 405 is often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM.

AZDye? 405 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AZDye? 405 is a bright, blue-fluorescent probe optimally excited by the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of moderate-abundance targets.

AZDye? 405 is structurally similar to Alexa Fluor? 405 or Cascade Blue, and spectrally is a match to DyLight? 405, Alexa Fluor? 405, CF? 405S Dye, Cascade Blue, or any other sulfonated pyrene based fluorescent dyes.

分子量
736.75 (protonated)
分子式
N/A
CAS
N/A
溶(解)度
Water, DMSO, DMF
純度
>90% (HPLC)
外觀
Yellow solid
儲存環境
-20°C. Desiccate
運輸條件
Ambient temperature

1. Schuler, D., et al. (2021). Differential Labeling of Chemically Modified Peptides and Lipids among Cyanobacteria Planktothrix and Microcystis. Microorganisms, 9, 1578. [MDPI]


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